Human IL-4
Interleukin-4 (IL-4) is a critical cytokine involved in humoral immunity and allergic inflammation. It is primarily secreted by T helper 2 (Th2) cells, as well as mast cells and basophils, and plays a key role in promoting Th2 differentiation, B cell proliferation, and immunoglobulin class switching to IgE and IgG. IL-4 exerts its effects by binding to its receptor complex (IL-4Rα paired with either the common gamma chain or IL-13Rα1), activating the JAK-STAT signaling pathway—particularly STAT6—to regulate gene expression and immune responses. Additionally, IL-4 suppresses Th1 responses, modulates inflammation, and supports tissue repair. When combined with GM-CSF, IL-4 drives monocyte differentiation into dendritic cells (moDCs), particularly in vitro.
Recombinant human IL-4 is widely used in vitro to investigate Th2-driven immunity, cytokine signaling, and gene regulation. It is a valuable tool in studying allergic diseases such as asthma and atopic dermatitis and is instrumental in drug discovery efforts targeting IL-4 pathways for immune modulation.
| Cat. No. | RC0004 |
|---|---|
| Purity | >95% in SDS-PAGE followed by coomassie staining |
| Endotoxin Level | <0.01 EU/µg (LAL Assay) |
| Source | Purified from the culture supernatant of HEK 293 cells genetically engineered to express human IL-4 protein with a C-terminal His tag. |
| Accession # | NP_000580 |
| Applications/Intended Use | Research Use Only: This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. |
| Molecular Mass | Predicted mass (15 kDa), appears 17-20 kDa due to glycosylation |
| Formulation | Lyophilized from a 0.1 μm filtered solution in PBS with Trehalose |
| Reconstitution | Dissolve in sterile PBS to 0.1-0.5 mg/mL or user preferred stock concentration. |
| Shipping or Handling Information | Shipped at ambient temperature |
| CoA | Batch-specific test results will be provided with the product |
| Stability | Lyophilized: Stable for 12 months at -80°C; Reconstituted: Stable for 2 weeks at 4°C, or 3-6 months at -20°C |
| Disclaimer | All of TME Scientific cell biology products are for research use ONLY and NOT for therapeutic/diagnostic applications. TME Scientific is not liable for any repercussions arising from the use of its cell biology product(s) in therapeutic/diagnostic or any other non-RUO application(s). |
