Human Peripheral Blood CD4+ T Cells

CD4+ T cells, also known as helper T cells, are effector cells for cell-mediated immunity. Following selection and maturation in the thymus, naïve CD4+ T cells migrate to the periphery where they survey for antigens displayed by human leukocyte antigen class II (HLA-II) molecules present on the surface of professional antigen presenting cells (APCs), such as Dendritic cells (DCs), B cells, and macrophages. If the antigen presented by the HLA-II molecules is recognized as foreign, then CD4+ T cells become activated leading to the production of cytokines to initiate immune responses from other white blood cells/other immune cells of cell-mediated immunity. Furthermore, they activate the T cell-dependent branch of humoral immunity, causing the activation of B cells to produce immunoglobulin in response to the antigen. As CD4+ T cells mediate anti-tumor immunity, they are critical to cancer immunotherapy research.

As a one-stop biomodeling service provider, TME offers CD4+ T-cell-based in vitro model systems:

1. To analyze the effect of food additives on T cell activation
2. To model CD4+ T cell polarization to assess a therapeutic’s ability to modulate T cell polarization away from pro-inflammatory phenotype and toward regulatory phenotype
3. To assess the impact of a xenobiotic on CD4 effector differentiation and activation state
4. To analyze CD4+ T cell exhaustion
5. To assess Treg expansion
6. To address the adjuvant effects of nanoparticles on the immune system based on an allogeneic co-culture model with human monocyte-derived DCs (MoDCs)
7. To analyze T-cell activation and proliferation (MLR assay)
8. For evaluating antigen specific responses
9. To evaluate CD4+T cell differentiation
10. To test the effect of immunomodulatory agents on T cell function and activation.
11. To measure vaccine immunogenicity using the co-culture of purified DC and naïve CD4+ T cells

CD4+ T cells were isolated using negative immunomagnetic separation techniques and Institutional Review Board (IRB)-approved protocols and consent forms were used to acquire cells.