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Human Peripheral Blood Mononuclear Cells (PBMCs)

$199.00

CAT: CPB0010

Peripheral blood mononuclear cells (PBMCs) constitute the cellular part of the blood and they play important roles in the immune system monitoring immune-relevant events. In humans, the frequencies of cell populations in PBMCs vary across individuals, but typically, lymphocytes are in the range of 70–90 %, monocytes between 10–20 %, and dendritic cells are rare, accounting for only 1–2 %. In recent years, PBMCs have received growing attention as surrogate markers of several diseases and they are widely used in research and clinical applications, such as immunotherapies, microbiology, virology, oncology, vaccine development, toxicology, as well as transplant and regenerative biology.

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CAT: CPB0010

Peripheral blood mononuclear cells (PBMCs) constitute the cellular part of the blood and they play important roles in the immune system monitoring immune-relevant events. In humans, the frequencies of cell populations in PBMCs vary across individuals, but typically, lymphocytes are in the range of 70–90 %, monocytes between 10–20 %, and dendritic cells are rare, accounting for only 1–2 %. In recent years, PBMCs have received growing attention as surrogate markers of several diseases and they are widely used in research and clinical applications, such as immunotherapies, microbiology, virology, oncology, vaccine development, toxicology, as well as transplant and regenerative biology.

CAT: CPB0010

Peripheral blood mononuclear cells (PBMCs) constitute the cellular part of the blood and they play important roles in the immune system monitoring immune-relevant events. In humans, the frequencies of cell populations in PBMCs vary across individuals, but typically, lymphocytes are in the range of 70–90 %, monocytes between 10–20 %, and dendritic cells are rare, accounting for only 1–2 %. In recent years, PBMCs have received growing attention as surrogate markers of several diseases and they are widely used in research and clinical applications, such as immunotherapies, microbiology, virology, oncology, vaccine development, toxicology, as well as transplant and regenerative biology.

Description

Peripheral blood mononuclear cells (PBMCs) constitute the cellular part of the blood and they play important roles in the immune system monitoring immune-relevant events. In humans, the frequencies of cell populations in PBMCs vary across individuals, but typically, lymphocytes are in the range of 70–90 %, monocytes between 10–20 %, and dendritic cells are rare, accounting for only 1–2 %. In recent years, PBMCs have received growing attention as surrogate markers of several diseases and they are widely used in research and clinical applications, such as immunotherapies, microbiology, virology, oncology, vaccine development, toxicology, as well as transplant and regenerative biology.

As PBMCs include various types of immune cells such as T cells, B cells and monocytes, PBMCs can be effectively used as an in vitro model system to study the interplay between these cell types which constitute a very important part of the peripheral immune system. Examining the collective effects on T cells, macrophages, and other cells from healthy donors provides a more informative approach compared to studying isolated cell cultures, as it closely models the in vivo functional immune response, thereby enhancing relevance to real-world scenarios.

As a one-stop biomodeling service provider, TME offers PBMC-based in vitro model systems:

  1. To test anti-inflammatory properties of compounds
  2. To predict the toxicity of various chemicals
  3. To develop platforms to test anti-inflammatory drugs
  4. To assess the effectiveness of anti-T cell immunotoxins
  5. To investigate how immunoregulatory monoclonal antibodies (mAb) can be used to facilitate or enhance a tumour-destructive immune response
  6. To assess the immunomodulatory function of adipose tissue-derived stromal cells (ASC)
  7. To test for drug-induced immune responses, or even to analyze biomaterial-drug interactions on the immune system
  8. To be used as in vitro T cell models of human naïve/memory T cell responses
  9. To model CD4+ T cell polarization to assess a therapeutic’s ability to modulate T cell polarization away from pro-inflammatory phenotype and toward regulatory phenotype
  10. To evaluate the function of CAR T cells by examining their effector functions
  11. To investigate cell interactions and the cellular immune response following stimulation by xenobiotics
  12. To examine the immune cell responses to oligonucleotides
  13. To detect innate immune response modulating impurities (IIRMIs) in therapeutic proteins
  14. To predict drug allergy
  15. To assess the immunogenic risk of a biotherapeutic
  16. To study the in vitro primary human NK cell cytotoxic activity
  17. To investigate how therapeutic antibodies–native and aggregated–influence DC cell maturation
  18. To mimic the immune response in the skin
  19. To detect contact skin sensitizers (alternative to animal testing)

PBMCs were isolated using density gradient separation techniques and Institutional Review Board (IRB)-approved protocols and consent forms were used to acquire cells.

Specifications
Cat. No. CPB0010
Category Primary Cells
Tissue Peripheral Blood
Donor History Healthy Donor
Growth Properties Suspension, Round
Applications/Intended Use Research Use Only: This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.
Volume/Unit 1 mL
Product Format Frozen
BioSafety II
Shipping or Handling Information Shipping with dry ice or liquid nitrogen
CoA Batch-specific test results will be provided with the product
Growth Conditions
  • IMDM + 10% FBS, or
  • DMEM w/ 4500mg/L D-Glucose + 10% FBS, or
  • RPMI 1640 Medium + 10% FBS
    • Cryopreservation Cryopreservation Medium (Cat. No. CT002)
    Disclaimer All of TME Scientific cell biology products are for research use ONLY and NOT for therapeutic/diagnostic applications. TME Scientific is not liable for any repercussions arising from the use of its cell biology product(s) in therapeutic/diagnostic or any other non-RUO application(s).